Contact an Account ManagerHPLC determination of ampicillin and its metabolites in rat plasma, bile and urine by postcolumn degradation with sodium hypochlorite
Summary
Type: HPLC
Application #: 103530
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 270nm, 0.032 AUFS (0 - 25 min), 0.64 AUFS (from 25 min)
Conditions: eluent: 10mM Na heptanesulphonate - 100mM H3PO4 - methanol (0.8:0.8:1, v/v)
flow rate 0.8ml/min
Authors: Haginaka, J. et al.
Source: J. Chromatogr. 400 (1987) 101–111
Details
Legend: Fig. 5. Chromatogram of 1 and its metabolites in rat urine after 10fold
dilution, A) pre-dose and B) 1 - 4 h post-dose urine samples treated as
described in the original paper, 20µl portion of the s